Two-Step Pretreatment of Lignocellulosic Biomass for High-Sugar Recovery from the Structural Plant Polymers Cellulose and Hemicellulose

Nitrogen explosive decompression pretreatment was tested on barley straw, aspen wood, and pine wood in a two-step approach. The approach aims to avoid the uncontrolled degradation of hemicellulose during recover high sugar yields from cellulose. Additionally, pretreated biomass filtered after each step with fine metal sieve so that fermentation hydrolysis inhibitors were removed. A total yield 43 g per 100 dry achieved straw wood. For significantly lower, 12 biomass. Possible reasons for this low are adsorption deactivation cellulases softwood repolymerized lignin pretreatment.